Technique Talk: Troubleshooting Quantitative PCR (qPCR)

In this workshop, you will learn about the critical factors that ensure qPCR accuracy and success.

From detecting trace levels of virus to quantifying oncogenes in patient samples, quantitative PCR (qPCR) is the cornerstone of many diagnostic tests. At the basic research-level, scientists rely on qPCR to evaluate changes in gene expression in varying conditions. qPCR success depends on careful primer design, sample and reagent purity, template composition, and experimental technique.

This Technique Talk will teach you the tips and tricks needed for obtaining perfect qPCR results.

Learning Objectives

• How to design and validate a qPCR assay
• Optimize qPCR-based analysis workflows
• Assess the performance of qPCR analysis