With over 40 years’ experience, Don Whitley Scientific Ltd (DWS) is the leading manufacturer and supplier of controlled atmosphere workstations to the scientific community.

DWS offers a wide range of hypoxic workstations, from small footprint personal workstations for those with space constraints, to extremely large workstations and multi-chamber units designed to house pieces of equipment.

DWS sells directly to UK customers and through carefully selected distributors elsewhere. HypOxygen has been our partner in the USA for over 30 years, servicing the whole of North America.

The DWS Hypoxic Workstation Range

Hypoxic workstations allow the study of cancer biology in a setting that accurately represents the oxygen deprived tumour microenvironment (TME). The O₂ concentration in Whitley Hypoxystations can range from 0.1 - 20% and this can be adjusted in increments of 0.1% allowing researchers to choose the O₂ concentration that best fits their needs. The tumour microenvironment consists of hypoxic and necrotic regions where the level of O₂ concentration can fluctuate. HIF-1α, which is normally broken down in the presence of O₂, is stabilised and well maintained in hypoxystations compared to conventional hypoxic incubators. The presence of HIF-1α upregulates vascular endothelial growth factors (VEGF) protein which promotes the formation of new vasculature to support the tumour cells with adequate levels of O₂ and nutrients. The new intricate blood supply that is formed is often disrupted and leaky and can therefore change the amount of O₂ received by the tumour. To mimic this, Don Whitley Scientific have developed O₂ profiling software, that allows you to rapidly cycle between different O­₂ concentrations within the desired time.

The DWS i2 Instrument Workstation

As part of our hypoxic range, DWS also offers the only workstation on the market specifically designed for housing Seahorse XF Analysers: the i2 Instrument Workstation. The combination of the i2 and Seahorse XF Analyzer permits simultaneous, real-time analysis of mitochondrial respiration and glycolysis in mammalian cells under precisely controlled hypoxic conditions. The i2 maintains an internal temperature no higher than 28°C and excludes CO₂. The i2 can also be attached to a Whitley Hypoxystation via a transfer tunnel. Here samples can be prepared/ manipulated in the Hypoxystation and passed through the tunnel into the i2 workstation for further sample processing using the Seahorse XF Analyser.

DWS also offers a range of other cell culture equipment, including hypoxic media conditioning systems, in-incubator microscopes, microfluidic devices, bioreactors, vacuum aspirators, 3D and perfusion culture systems.

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www.dwscientific.com

www.HypOxygen.com