Click on the 'Resources' tab to download the ePoster. 

Role of PGE2 on Neutrophils during Human Tuberculosis

Martin C 1;2 , Pellegrini JM 1;2 , Morelli MP 1;2 , Tateosian NL 1;2 , Amiano NO 1;2, Ciallella L 3 ,Palmero DJ 3 , García VE 1;2 .

1 Departamento de Química Biológica. Facultad de Ciencias Exactas y Naturales. UBA, Intendente Güiraldes 2160, Pabellón II, 4°piso, Ciudad Universitaria (C1428EGA), Buenos Aires, Argentina.

2 Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN). Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Intendente Güiraldes 2160, Pabellón II, 4°piso, Ciudad Universitaria (C1428EGA), Buenos Aires, Argentina.

3 División Tisioneumonología Hospital F.J. Muñiz, Uspallata 2272, (C1282AEN) Buenos Aires, Argentina.

Tuberculosis (TB) is one of the top 10 causes of death worldwide. Prostaglandin E2 (PGE2), an active lipid compound derived from arachidonic acid, regulates different stages of the response of the host during several pathologies such as chronic infections or cancer. Interestingly, manipulation of PGE2 levels was proposed as an approach for countering the Type I IFN signature of TB, although very limited information about this pathway in tuberculosis patients is available. Therefore, the aim of the present work was to investigate the role of this compound during human TB.

Neutrophils were obtained from heparinized peripheral blood from healthy donors (HD) and tuberculosis patients (TB) by Ficoll-Hypaque centrifugation and Dextran sedimentation. Cells were cultured (2x10ˆ6 cells/ml) with a Mycobacterium tuberculosis lysate (Mtb-Ag, 10μg/ml) with/without PGE2. Then, we evaluated the role of this lipidic mediator during the human immune response against Mtb-Ag by using flow cytometry and confocal microscopy assays. P-values xtagstartz0,05 were considered significantly different.