Evaluation of the antidiabetic properties of Gongronema latifolium leaves extract-loaded solid lipid microparticles Salome A. Chime, Ph.D1,*; Paschal U. Ugwoke, B. Pharm.1; Anthony A. Attama, Prof. of Pharmaceutics 2 1Department of Pharmaceutical Technology and Industrial Pharmacy;2 Department of Pharmaceutics, University of Nigeria, Nsukka 410001, Nigeria. Context: Gongronema latifolium Benth Hook, (Asclepiadaceae) is a tropical rainforest plant primarily used in traditional folk medicine in the treatment of malaria, diabetes, hypertension, and as laxative. Aim: To study the antidiabetic properties of Gongronema latifolium-loaded solid lipid microparticles (SLMs). Materials and Methods: The methanolic extract of Gongronema latifolium leaves were obtained and formulated into SLMs using lipid matrix comprising of fat from Capra hircus and Phospholipon® 90H. The SLMs were characterised in vitro by determining the particle size and morphology, pH stability studies and encapsulation efficiency EE%. Also the antidiabetic properties of the Gongronema latifolium-loaded SLMs were studied in alloxan-induced diabetic rats. Results and discussion: The results show that particle size of G. latifolium-loaded SLMs were within acceptable range for SLMs. Highest EE% of 68 % was obtained for SLMs containing 5 % G. latifolium (batch F3). The formulations remained stable with insignificant pH reduction over time (p < 0.05). Gongronema latifolium-loaded SLMs had mean percentage reduction in blood glucose of 76 % at 2 h, 42.3 % at 8 h and 24.4 % at 12 h, while the group that received the reference-glibenclamide had 82.6, 61.7 and 46.7 % at 2, 8 and 12 h respectively after oral administration of all samples. Gongronema latifolium-loaded SLMs had blood glucose reduction significantly higher than the pure extract and the glibenclamide (p < 0.05) at the concentrations used. Conclusion: Gongronema latifolium-loaded SLMs exhibited good hypoglycemic effect and could be used for the treatment of diabetes.
Department of Pharmaceutical Technology and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Nigeria
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