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Obesity: From Cell to Patient | EK17


EK17-eposter-botha-danelle - Peripheral Effect of Adipogenesis on ATM Expression and Mirna-181b Levels in Cardiomyoblasts


Feb 1, 2021 12:00am ‐ Feb 1, 2021 12:00am

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Peripheral Effect of Adipogenesis on ATM Expression and Mirna-181b Levels in Cardiomyoblasts Danélle Botha (a), Marguerite Blignaut (a), Hanél Sadie-van Gijsen (a), Paul van Jaarsveld (b), Barbara Huisamen (a) (a) Centre for Cardio-metabolic Research in Africa (CARMA), Division of Medical Physiology, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, South Africa. (b) Non-Communicable Diseases Research Unit (NCDRU), South African Medical Research Council (SAMRC). (19198639@sun.ac.za) Obesity is a risk factor for metabolic abnormalities that precede type II diabetes and cardiovascular diseases. Adipose tissue communicates with peripheral organs through endocrine secretions, which become dysregulated during obesity. In turn, dysregulated adipose secretions may influence the protein levels of metabolic sensor proteins, including mammalian target of rapamycin complex 1 (mTORC1) and AMP-activated protein kinase (AMPK), in peripheral tissues. Ataxia-telangiectasia mutated kinase (ATM) regulates glucose and redox homeostasis and is decreased in muscle and cardiac tissues of obese and insulin resistant animals. ATM down-regulation therefore represents a potential link between obesity and cardiac metabolic dysfunction, although the underlying mechanism is unknown. MicroRNAs (miRNAs) regulate protein translation in health and disease and are a potential mechanism for ATM regulation in obesity. Thus, this study investigated the effect of in vitro secretions in conditioned media (CM) from adipose stem cells (ASCs) and their differentiated adipocyte counterparts obtained from the subcutaneous and visceral fat depots of control (n=4) and high-fat diet (HFD, n=4) male Wistar rats on i) ATM, mTORC1 and AMPK levels and activity (Western blot) and ii) levels of miR-181b that targets ATM in silico (qRT-PCR) in H9c2 cardiomyoblasts. The CM fatty acid composition was determined with GC-FID. Adipocytes secreted higher levels of palmitic acid (p

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