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eSymposia | Harnessing the Microbiome for Disease Prevention and Therapy


Chitosan-based microparticle encapsulated Acinetobacter baumannii phage cocktails in hydrogel matrix for management of drug resistant chronic wounds


Jan 18, 2021 12:00am ‐ Jan 18, 2021 12:00am

Description

Chitosan-based microparticle encapsulated Acinetobacter baumannii phage cocktails in hydrogel matrix for management of drug resistant chronic wounds Abstract Background: Phage therapy is seen as a possible treatment option in the management of multi-drug resistant strains of many bacteria. This study seeks to formulate a chitosan-based hydrogel containing microparticle encapsulated bacteriophage for in vivo wound healing evaluation in a drug resistant chronic animal wound model. Method: Microparticle entrapped phage cocktails were evaluated for their ability to treat and completely resolve Acinetobacter baumannii and Pseudomonas aeruginosa induced diabetic wound infection. The bacteriophage cocktail was entrapped in the microparticle and suspended in a hydrogel matrix. Wound healing was assessed and histomorphometry values were used as determinants for wound resolution in conjunction with measurement of wound closure and absence of infection at injury site. Results: The percentage wound contraction of hydrogels loaded with the phage particles were 74.56% ± 1.74% compared to the control without treatment and control treatment with conventional gentamicin ointment 18.45% ± 2.03% and 30.1% ± 0.93% respectively. Number of inflammatory cell infiltrates were statistically lower in the animals that were treated with phage hydrogels 102 ± 3 cells/mm2 of the filed compared to the control without treatment 1300 ± 12 cells/mm2. Conclusion: Chitosan-based microparticle encapsulated Acinetobacter baumannii phage and Pseudomonas aeruginosa bacteriophage in hydrogel matrix showed a positive response towards the healing of the wound when compared to the positive control. This can be a more effective way to reduce the rate of antimicrobial resistance in diabetic wound healing. Authors: Margaret O. Ilomuanya1 milomuanya@unilag.edu.ng 0000-0001-8819-1937 Nkechi V. Enwuru2 nenwuru@unilag.edu.ng 0000-0003-2285-0682 Emmanuella Adenokun1 nuel_eo@yahoo.com Abigail Fatumbi1 pharmhabby@gmail.com Adebowale Adeluola2 aadeluola@unilag.edu.ng 0000-0002-7235-6052 Cecilia I. Igwilo1 cigwilo@unilag.edu.ng 0000-0002-7950-7714 1 Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy. University of Lagos, PMB 12003, Surulere, Lagos, Nigeria 2 Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy. University of Lagos, PMB 12003, Surulere, Lagos, Nigeria Key words: Bacteriophage, Chitosan, Encapsulation, Wound healing, Hydrogels. *Corresponding Author: milomuanya@unilag.edu.ng

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