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eSymposia | Harnessing the Microbiome for Disease Prevention and Therapy


Gut Dysbiosis in Patients with Pulmonary Arterial Hypertension


Jan 18, 2021 12:00am ‐ Jan 18, 2021 12:00am

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Gut Dysbiosis in Patients with Pulmonary Arterial Hypertension Daphne Moutsoglou1, Matthew Hamilton2, E. Kenneth Weir3, Alexander Khoruts1, Thenappan Thenappan3 1. Division of Gastroenterology, Hepatology, and Nutrition. Department of Medicine. University of Minnesota, Minneapolis. 2. Cardiovascular Division, Department of Medicine. University of Minnesota, Minneapolis 3. Biotechnology Institute. College of Biological Sciences, University of Minnesota, Minneapolis. Background: The pathophysiology of pulmonary arterial hypertension (PAH) is poorly understood but may occur from remodeling of the extracellular matrix with fibrosis of pulmonary vessels that is primarily driven by perivascular inflammation and immune dysregulation. Recent studies suggest a role for the gut-pulmonary axis in pulmonary and cardiovascular disease. Hypothesis: We hypothesized that gut dysbiosis and barrier dysfunction lead to an altered burden of circulating microbial metabolic products and bacterial components, resulting in perivascular inflammation that promotes PAH. Methods: DNA was isolated from the fecal samples of 35 PAH patients and 36 healthy controls, and the V4 hypervariable region of the 16S rRNA gene was amplified and sequenced using the Illumina platform. Sequences were analyzed using mothur software (open source, www.mothur.org) and Linear discriminant analysis Effect Size (LEfSe). Results: On principal component analysis, patients with PAH had a distinct intestinal microbiome composition compared to healthy controls (Figure 1A). Additionally, the Shannon diversity index was significantly lower in PAH patients (3.394 ± 0.08 vs. 3.938 ± 0.04, p

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