Vibrio cholerae OmpU induces activation of dendritic cells via NLRP3 inflammasome and TLR2 Vinica Dhar and Arunika Mukhopadhaya Indian Institute of Science Education and Research (IISER), Mohali-140306 email@example.com, firstname.lastname@example.org OmpU, an outer membrane porin protein of gram-negative human pathogen Vibrio cholerae, is highly expressed during pathogenesis. Our lab has reported that OmpU can activate innate immune cells, such as, monocytes and macrophages. The purpose of this study is to explore whether OmpU can modulate dendritic cell (DC) responses and the underlying mechanism of activation. DCs being a crucial cell type decides the fate of adaptive responses acting as an intermediary between host innate and adaptive immune system. We observed that OmpU can activate DCs (bone-marrow derived and splenic DCs) in terms of production of pro-inflammatory mediators such as, TNFα, IL-6, IL-1β and ROS in TLR2 dependent manner. Our study also revealed that OmpU-mediated pro-inflammatory responses involve MAP kinases, PI3K and PKC. Additionally, calcium signalling and mitochondrial ROS are involved in the activation of NLRP3 inflammasome which results in IL-1β production.
Indian Institute of Science Education and Research (IISER), Mohali
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