SeqScreener: a cloud-based software application for analyzing CRISPR experiments with Sanger Sequencing data Genome editing is poised to revolutionize the life sciences industry due to its precise changes at locus using CRISPR-Cas9 or other nucleases. SeqScreener was released to serve customers with data analysis of gene edit confirmation. Since the CRISPR repair process is not completely accurate, the pool of gene-edited cells needs rounds of screenings to become homogeneous. Pooled screen is to determine the relative fraction of desired edits. After cell expansion, a clonal screen finds clonally pure cells. As a critical step in screening protocols, Sanger sequencing is the most cost-effective measurement method with fast turnaround time to reveal the details around CRISPR cut site and the abundancy of each edited gene. Thermo Fisher Scientific had introduced True Design Genome Editor software for experiment designs. For data analysis, the SeqScreener Gene Edit Confirmation tool deconvolves the sequence trace of the mixture of wild-type and mutated sequences, generating results of gene editing efficiency, frame shift percentage, diversity of edits, and indel spectrum. It completed the Themo Fisher Scientific gene editing end-to-end workflow along with other gene editing products. Seqscreener has both pooled screening and clonal screening workflows. Its processing speed is 6.5 faster than competitors. It has clear visualization tools and the unique gene editing sample ranking system, which helps customers to select clones efficiently. SeqScreener also has capability to suggest related gene editing products to customers based on detected sample quality. The future plan is to provide “hands-free” solutions such that sequencing data will be auto-uploaded directly from CE instruments and get analyzed within minutes without manual steps. SeqScreener simplifies data analysis and empowers the use of Sanger Sequencing as a genome editing confirmation tool.