Using mitochondrial TOP1 (TOP1mt) to probe the involvement of mitochondria in Systemic Lupus Erythematosus Hongliang Zhang1, Mariana Kaplan2, and Yves Pommier1 1Developmental therapeutics Branch, CCR, NCI 2Systemic Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, NIH
Systemic lupus erythematosus (SLE), also known simply as lupus, is a chronic, multisystem autoimmune disorder. The standard lupus tests of anti-nuclear antibodies and anti-dsDNA antibodies establish the central role of nuclear components in the onset of lupus symptoms. Since both nuclear and mitochondrial domains contain DNA, we investigated the involvement of mitochondria in lupus. We take advantage of the facts that both nuclei and mitochondria have their own topoisomerase I—TOP1 and TOP1mt, respectively. We screened the lupus sera against TOP1 and TOP1mt with Enzyme-linked immunosorbent assay (ELISA). With the confirmation of Western blots, it is TOP1mt not TOP1 reacted to the lupus sera, which pointed out the importance of mitochondria in the process of lupus. We speculate that mitochondria play a to-be-realized important role in lupus etiology.
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