Induced MHCII expression on breast cancer cells broadens the responding T cell repertoire, delays tumor-specific T cell exhaustion, and impairs tumor growth
Tyler R. McCaw*1; Selene Meza-Perez1; Mei Li2; Donald J. Buchsbaum2; Dmytro Starenki3; Sara Cooper3; Andres Forero1; Troy D. Randall1
1Department of Medicine, Univ. of Alabama at Birmingham, Birmingham, AL; 2Department of Radiation Oncology, Univ. of Alabama at Birmingham, Birmingham, AL; 3HudsonAlpha Institute for Biotechnology, Huntsville, AL
We recently reported Major Histocompatibility Class II (MHCII) expression on human triple negative breast cancer cells correlates with prolonged progression-free survival and increased tumor infiltrating lymphocytes. We hypothesized that expression of MHCII enhanced the intratumoral CD4+ T cell response, thereby bolstering the tumor-specific CD8+ T cell response. To test this we transfected murine breast cancer (TS/A) cells with the human MHCII transcriptional activator (hCIITA) or empty vector, injected transfected cells into BALB/c mice, and analyzed the evolving immune response. We found hCIITA-expressing tumors grew much slower than controls in immunocompetent mice, but this difference was nullified in immunocompromised and markedly reduced in CD4+ T cell depleted mice. CD4+ T cells isolated from hCIITA-transfected tumors produced more IFNγ, IL-17A, and surprisingly granzyme B for longer times than their counterparts in control tumors. Similarly, CD8+ T cells from hCIITA-transfected tumors were more activated and produced more IFNγ and granzyme B for longer times. Nevertheless, both CD4+ and CD8+ T cells eventually became exhausted in both groups. TCR repertoire analysis showed both the breadth and magnitude of expansion of responding clones were increased in hCIITA-transfected tumor infiltrating T cells. Collectively, these results suggest that expression of MHCII on tumor cells promotes local CD4+ T cell effector functions, enhancing CD8+ T cell responses and delaying T cell exhaustion. Thus, epigenetic modifiers, like histone deacetylase inhibitors, that induce MHCII expression on tumor cells will likely benefit anti-tumor immune responses.
Credits: None available.
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