A Vesicles trafficking between cytoplasm and mitochondria
Chun-Hong Chen NHRI, Taiwan
Mitochondria are highly dynamic organelles that frequently undergo fusion and fission. Mitochondria are also involved in cell signaling pathways, including cell death, and autophagy. but how the cell signaling deliver from cell membrane into mitochondria still remain unclear. Recently one mitochondrial‐derived vesicles (MDVs) has been uncovered; it suggests there is indeed a vesicle trafficking pathway linking out from mitochondria to other cellar organelles. We investigated whether cytoplasmic vesicles are transported into the mitochondria in Drosophila model, as well as the potential mechanism by which the vesicle uptake occurs. We found that the overexpression of the mitochondria-associated protein, Enlarged and Clustering Mitochondria (ECM), caused mitochondrial enlargement and clustering, and these enlarged mitochondrial uptake of novel double membrane vesicles. We propose that the enlargement of mitochondria in cells overexpressing ECM is caused by the uptake these double-membrane vesicles. The electron microscope (EM) results showed these enlarged and cluster mitochondria contain double membrane vesicles. From immune gold staining, these vesicles could engulf the cytoplasmic protein into the vesicle. And mitochondria associated Rab protein Rab32 and the member of autophagy initiation complex Atg14 are involved in this process. In this talk, we will address the function and potential mechanism of these mitochondria vesicles, and the impact of cellular physiology, including aging and neuron degeneration diseases.
Credits: None available.
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