Quantitative Multi-Analyte Detection of Key Cytokine Release Syndrome Biomarkers: Workflow Optimization with an Automated, Microfluidic Immunoassay System Greta Wegner, Rainer Grant, Samuel Thayer, Samuel Leske, Michael Anderson, Tami Munn, Kathy Brumbaugh BioTechne, Minneapolis,MN 55413 USA
We designed a quantitative multi-analyte detection assay of key cytokine release syndrome (CRS) biomarkers. This microfluidic parallel immunoassay measured four cytokines simultaneously in only one hour. We compared cytokine measurements for human IL-6, IL-1β, TNF-α, and IFN-γ between Quantikine ELISA and Simple Plex immunoassays using an in vitro model for CRS. Samples from all donors used in this in vitro model showed similar results across both immunoassay platforms. The large dynamic ranges of the Simple Plex assays allowed for monitoring of baseline sub-picogram cytokine levels in untreated samples. This automated Simple Plex assay panel optimizes lab workflow from screening to precise detection of cytokines associated with CRS and other processes where monitoring immune responses accurately and quickly is essential.
Credits: None available.
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