Chewing the Fat: Panel and Discussion about Autophagy and Future Directions of the Autophagy Field
Keystone Symposia ePanel:
Chewing the Fat: Panel and Discussion about Autophagy and Future Directions of the Autophagy Field
This panel discussion occurred during the "Autophagy: Molecular and Physiological Mechanisms" Keystone Symposia conference in Whistler, British Columbia, Canada on June 7, 2016. Eric Baehrecke, Ana Maria Cuervo, Vojo Deretic and Leon Murphy met to discuss their interests in autophagy. Topics included some history, current and future areas of research focus, controversies, and speculation about the future directions of the autophagy field.
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About Keystone Symposia:
Keystone Symposia is a 501(c)(3) nonprofit organization founded in 1972 that convenes 50-60 open, international scientific research conferences each year across the full range of the life sciences – from cardiovascular disease to immunology to neurobiology. The conferences accelerate life science discovery by bringing together and fostering collaboration among the world’s leading and next generation of research scientists.
Website: www.keystonesymposia.org
Moderator(s):
- Eric H. Baehrecke, PhD, University of Massachusetts Medical School
Panelist(s):
- Ana Maria Cuevo, MD, PhD, Albert Einstein College of Medicine
- Vojo Deretic, PhD, University of New Mexico Health Sciences Center
- Leon O. Murphy, PhD, Casma Therapeutics
Autophagy: Controversies, Challenges & Opportunities
Keystone Symposia ePanel:
Autophagy: Controversies, Challenges & Opportunities
Recorded during Keystone Symposia meeting: Autophagy [E615]
Date: June 19 - 24, 2015
Location: Beaver Run Resort, Breckenridge, CO
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About Keystone Symposia:
Keystone Symposia is a 501(c)(3) nonprofit organization founded in 1972 that convenes 50-60 open, international scientific research conferences each year across the full range of the life sciences – from cardiovascular disease to immunology to neurobiology. The conferences accelerate life science discovery by bringing together and fostering collaboration among the world’s leading and next generation of research scientists.
Website: www.keystonesymposia.org
Moderator(s):
- Eric H. Baehrecke, PhD, University of Massachusetts Medical School
Panelist(s):
- Ana Maria Cuevo, MD, PhD, Albert Einstein College of Medicine
- Vojo Deretic, PhD, University of New Mexico Health Sciences Center
- Leon O. Murphy, PhD, Casma Therapeutics
Optimal Combinations of Broadly Neutralizing Antibodies for Prevention & Therapy of HIV-1 Clade C Infection
Keystone Symposia SciTalks:
Optimal Combinations of Broadly Neutralizing Antibodies for Prevention & Therapy of HIV-1 Clade C Infection
Recorded: March 20 - 24, 2016
Keystone Symposia meeting: HIV Vaccines [X8]
Location: Resort at Squaw Creek, Olympic Valley, CA
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About Keystone Symposia:
Keystone Symposia is a 501(c)(3) nonprofit organization founded in 1972 that convenes 50-60 open, international scientific research conferences each year across the full range of the life sciences – from cardiovascular disease to immunology to neurobiology. The conferences accelerate life science discovery by bringing together and fostering collaboration among the world’s leading and next generation of research scientists.
Website: www.keystonesymposia.org
Speaker(s):
- Kshitij Wagh, PhD, Los Alamos National Laboratory
Why Mosquitoes Matter
Recorded on February 19, 2017
Pre Meeting Workshop
Malaria: From Innovation to Eradication
Feb 19 - Feb 23, 2017 | Kampala, Uganda
View and Download Presentation
This Keystone Symposia SciTalk was made possible by a grant from the
The goal of the pre meeting workshop is to provide valuable background on what to expect for the upcoming Keystone Symposia. The intent is to bring participants up to speed on the concepts to be presented at the meeting and enable the them to maximize their experience.
Speaker(s):
- Krijn Paaijmans, PhD, ISGlobal, Barcelona Institute for Global Health
Broadly Neutralizing Antibodies to HIV: History, Challenges and Hopes
Recorded on March 26, 2017
Pre Meeting Workshop
HIV VACCINES
March 26 - March 30, 2017
Steamboat Springs, Colorado, USA
This Keystone Symposia SciTalk was made possible by a grant from the
The goal of the pre meeting workshop is to provide valuable background on what to expect for the upcoming Keystone Symposia. The intent is to bring participants up to speed on the concepts to be presented at the meeting and enable the them to maximize their experience.
Recorded on March 26, 2017
Speaker(s):
- Elise Landais, PhD, IAVI Neutralizing antibody Center
At the edge of survival: Genetic associations for cell death in pemphigus foliaceus
Speaker(s):
- Angelica B. Boldt, PhD, Universidade Federal do Paraná
Developing a collaborative network to study TB and HIV biomarkers
Developing a collaborative network to study TB and HIV biomarkers
Dr. Andrade lab is dedicated to identifying reliable host biomarkers that can predict susceptibility to infection and disease severity in tuberculosis (TB) and HIV. By establishing a strong network of collaborators from different TB endemic countries, such as India, China, South Africa and Brazil, his group has designed cohort studies in which synchronization of clinical and immunologic data collection results in validation of biomarker findings across countries. In addition, the Andrade Lab performs integrative analyses merging epidemiologic, socioeconomic, clinical, immunologic, microbiologic and geographic data to more robustly describe the TB determinants and help to delineate more reliable decision-making strategies.
Speaker(s):
- Bruno B. Andrade, MD, PhD, Oswaldo Cruz Foundation (FIOCRUZ)
Development of CAR-T Cell Therapies for Leukemia
Speaker(s):
- Martin Bonamino, Brazilian National Cancer Institute
Tumor neoantigens identificsation and formulation testing for therapeutic vaccines development to treat cancer
Speaker(s):
- Patrícia Neves, Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos)
Waiting to die: Signalosomes kinetically control cell fate
Alejandro Rodriguez Gama1, Tejbir Kandola1, Shriram Venkatesan1, Jianzheng Wu1,2, Minling Hu1, and Randal Halfmann1,2
1Stowers Institute for Medical Research, Kansas City, MO, USA,
2Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, USA
Multiple signaling proteins of the innate immune system exert their cellular activities by assembling into large macromolecular complexes known as signalosomes. We previously discovered that two such proteins – the pyroptosome scaffold ASC, and the antiviral signaling protein MAVS – each assemble through self-templating polymerization that is reminiscent of infectious protein particles known as prions 1. More recently we revealed that prion-like activity broadly arises from structurally encoded kinetic barriers to nucleation – the probabilistic formation of a self-templating multimer de novo 2. For proteins like MAVS and ASC, the nucleation barrier is so high that their soluble inactive states persist despite physiological concentrations that are highly supersaturated with respect to the assembled active state. To identify other innate immune signaling proteins that may function in this manner, we used DAmFRET, a flow cytometric cell-based assay of nucleation barriers 2, to screen 138 candidate prion-like modules from 129 human proteins that function in programmed cell death and innate immune signaling. We discovered 36 of these proteins that are inherently capable of supersaturation and switch-like self-templating activation in living cells. We have further discovered a network of nucleating interactions between them, wherein polymerization of one protein nucleates the polymerization of specific additional proteins. This widespread kinetic control over cell fate indicates that cells are literally waiting to die -- pyroptosis, necroptosis, and alternative cell fates downstream of these proteins are thermodynamically favored, and therefore inevitable with time. I will discuss our investigations into the implications of this phenomenon for aging-associated inflammation and innate immune memory in human monocytes.
1. Cai, X. et al. Prion-like polymerization underlies signal transduction in antiviral immune defense and inflammasome activation. Cell 156, 1207–1222 (2014).
2. Khan, T. et al. Quantifying Nucleation In Vivo Reveals the Physical Basis of Prion-like Phase Behavior. Mol. Cell 71, 155-168.e7 (2018).
Speaker(s):
- Randal Halfmann, Stowers Institute