The expression of Pink1 and parkin in the mouse uterus during the estrous cycle Minha Cho, Sohyeon Moon1, Sangho Lee1, Sujin Lee1, Miseon Park, Minhwa Do1, Hoon Jang1, jinseop Ahn1, Okhee Lee1, Youngsok Choi1,2 1Department of Biomedical Science, CHA University, Seoul, Republic of Korea; 2Fertility Center of CHA Gangnam Medical Center, Seoul, Republic of Korea
PTEN-induced putative kinase 1(Pink1) is a mitochondrial serine/threonine kinase encoded by the PINk1 gene and Parkin is E3 ubiquitin ligase that plays a important role in ubiquitination. These two proteins are involved in a common pathway regulating mitochondrial quality control and promoting the selective autophagy of depolarized mitochondria (mitophagy). Mitophagy has been recently suggested to play critical roles in terminal differentiation of red blood cells, paternal mitochondrial degradation, neurodegenerative diseases, and ischemia or drug-induced tissue injury. However, there has been no report on the influence of pink1, parkin-mediated mitophagy in the uterus during the estrous cycle. In this study, we investigated the expression of pink1 and parkin in the mouse uterus during the estrous cycle using reverse transcription polymerase chain reaction(RT-PCR), quantitative real-time polymerase chain reaction(qRT-PCR), immunostaining. Pink1 and parkin mRNA was highly expressed in mouse kidney, heart, brain and also expressed in mouse reproductive tissues, including the uterus. The expression level of Pink1 and parkin mRNA was slightly increased at the stage of proesturs and diestrus of the cycle, compared with that at estrus and metestrus stage. These proteins highly located in stromal cell of the uterus at estrus and diestrus of the estrous cycle. We observed that the mRNA expression and location of Pink1 and parkin were regulated in the uterus during the estrous cycle. It needs further study to look into which affect temporally and spatially the expression of pink1 and parkin in mouse uterus.
References 1)Truban D et al: PINK1, Parkin, and Mitochondrial quality control: what we learn about Parkinson's Disease Pathobiology? J parkinsons Dis 2017;7;13-29 2) Seirafi M et al: Parkin structure and function. The FEBS Journal 2015;282;2076-2088 3) Ding WX, Yin XM : Mitophagy: mechanisms, pathophysiological roles, and analysis. Biol Chem 2012;393;547-564
Funding This research was supported by BK21 PLUS program (22A20130012771) through the NRF funded by the ministry of Education, Korea