Assaying the role of mitochondria dynamics during dehydration/rehydration of yeast cells
Chang-Lin Chen, Chuang-Rung Chang Institute of Biotechnology, National Tsing-Hua University, Hsinchu, Taiwan Saccharomyces cerevisiae is one of few anhydrobiotic organisms on earth capable of resisting dehydration process. Several stressful effects are harmful for cell survival during dehydration, including osmotic, oxidative, and mechanical constraints. In order to overcome these constraints, yeast cells have protective mechanisms for their desiccated survival. Previous studies indicated that protecting oxidative phosphorylation machinery is essential for better desiccation tolerance. However, detail mechanisms remain to be clarified. Mitochondria are dynamic organelles undergo fusion and fission process to maintain its integrity for energy production and inheritance. In this study, we aim to clarify the role of mitochondrial fusion/fission in yeast survival during dehydration/rehydration processes. Our results indicate that mitochondrial dynamics is involve in desiccation tolerance. This may derived from well-regulated dynamics is required for maintaining mitochondrial DNA. These results demonstrated that mitochondria dynamics machinery contributes to desiccation tolerance in yeast cells.