mTOR and Lysosomes in Growth Control David M. Sabatini Whitehead Institute for Biomedical Research, Cambridge, MA, USA
Our lab is interested in the regulation of growth and metabolism by nutrients and for some time we have focused on the mTOR pathway, particularly the nutrient-sensing network anchored by mTOR Complex 1 (mTORC1). I will discuss our latest work on how mTORC1 senses cytosolic and lysosomal amino acids and the role selective autophagy plays. I will highlight our use of a method we developed to profile the metabolite and protein content of organelles to identify proteins that move on and off lysosomes in response to nutrient conditions. I also may present our use of somatic cell genetics to identify new components of metabolic pathways, particularly in mitochondrial one-carbon metabolism.