Speciation Studies Investigating the Active Vanadium Compound Exerting the Immuno-moduling Effects on Oncolytic Viruses
Debbie C.Crans*1, Heide Murakami1, Mohammed Selman2, Anabel Bergeron2, Jean-Simon Diallo2
1Department of Chemistry, Colorado State University, Fort Collins, CO, USA; 2Department of Biochemistry, Microbiology and Immunology, Centre for Innovative Cancer Research, Ottawa Hospital Research Institute, Ottawa, ON, Canada
*Corresponding Author (email@example.com)
Oncolytic viruses (OV) are an emerging class of anticancer bio-therapeutics that induce antitumor immunity through selective replication in tumor cells. To improve the limited effects of OVs as single agents we have introduced a strategy to boost their efficacy (1, 2). Using immuno-modulating, small molecule protein tyrosine phosphatase inhibitors, specifically vanadium compounds, we are able to enhance the OV infection in vitro and ex vivo, in resistant tumor cell lines. The following abstract is focused on investigation of the nature of the vanadium compound exerting the immune-modulating effect. The approach taken is to examine the chemistry that vanadium undergoes under physiological conditions. Specifically, chemical speciation studies (2) are used to investigate how effects are changed when the different form of vanadium compounds are tested. The results show that under the conditions of the studies, oxovanadates interconvert resulting in very similar effects. These studies are discussed considering that vanadyl sulfate is more readily processed and less toxic.
(1) “Multi-Modal Potentiation of Oncolytic Virotherapy by Vanadium Compounds” Mohammed Selman, Christopher Rousso, Anabel Bergeron, Hwan Hee Son, Ramya Krishnan, Nader A. El-Sayes, Oliver Varette, Andrew Chen, Fanny Tzelepis, John C. Bell, Debbie Crans, and Jean-Simon Diallo, Molecular Therapy 2018, 26, 1, 1-14. ((2017), https://doi.org/10.1016/j.ymthe.2017.10.014)
(2) “Metal speciation in health and medicine represented by iron and vanadium,” Debbie C. Crans, Kellie A. Woll, Kestutis Prusinskas, Michael D. Johnson, and Eugenijjus Norkus, Inorg. Chem., 2013, 52, 12264-12275.