Description

Revealing Novel Cell Types, Cell-Cell Interactions, and Cell Lineages by

Single-Cell Sequencing

Alexander van Oudenaarden1,2

1Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences),Utrecht, the Netherlands; 2University Medical Center Utrecht, Cancer Genomics Netherlands, Utrecht, the Netherlands

In this talk I will report on our progress on detecting new cell types, cell-cell interactions, and cell lineages using single-cell sequencing. I will present three recent approaches, RaceID, StemID, and ProximID, that are used to detect rare cells, stem cells, and cell-cell interactions respectively. These algorithms are applied to several experimental model systems including the mammalian intestine, pancreas, and bone marrow. I will also present new data on detecting 5-hydroxycytosine methylation (5hmC) in single cells. This method does not only reveal extensive cell-to-cell heterogeneity of this epigenetic mark but also provide a powerful tool to perform endogenous lineage tracing at the single cell level.